【文獻標題】Novel ultrastructural findings on cardiac mitochondria of huddling Brandt＇s voles in mild cold environment

【作者】Zhe Wang，Jin-hui Xu，Jun-jie Mou，et.al

【作者單位】曲阜師范大學（Qufu Normal University）

【文獻中引用產(chǎn)品】

田鼠三磷酸腺苷（ATP）elisa試劑盒

田鼠檸檬酸合成酶（CS）ELISA試劑盒

田鼠半胱氨酸蛋白酶3（caspase3）ELISA試劑盒

【關(guān)鍵詞】huddling, apoptosis, mitochondrial fission, low temperature, heart, mitochondrial auhagy

【DOI】https://doi.org/10.1016/j.cbpa.2020.110766

【影響因子(IF)】4.0

【出版期刊】《Comparative Biochemistry ａnd Physiology, Part A》

【產(chǎn)品原文引用】

ATP synthase, CS, ａnd caspase3 activity

Samples stored at 80 °C were used to detect ATP synthase, citrate synthase, ａnd caspase3 activity. ATP synthase activity was determined by measuring free phosphate group at 450 nm using an ATP synthase Activity Assay Kit (H-172421, Shanghai Hengyuan Biological Technology Co., Ltd., China) according to the manufacturer’s instructions (OuYang et al., 2018).Citrate synthase activity was determined by measuring coenzyme A formation at 450 nm with a Citrate Synthase Activity Assay Kit (H-109821, Shanghai Hengyuan Biological Technology Co., Ltd., China) according to the manufacturer’s instructions (Song et al., 2018). Caspase3 activity in cell lysates was determined using a Caspase3 Activity Kit (BC3830, Solarbio, Beijing, China) following the manufacturer’s protocols (Xu et al., 2018).

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原創(chuàng)作者：上海恒遠生物科技有限公司